"GEMINI leverages a computationally designed protein assembly as an intracellular memory device to record the history of individual cells. GEMINI grows predictably within live cells, capturing cellular events as tree-ring-like fluorescent patterns for imaging-based retrospective readout. Absolute chronological information of activity histories is attainable with hour-level accuracy."
"Cells constantly change their molecular state in response to internal and external cues. Mapping cellular activity in tissues with spatiotemporal precision is essential for understanding organ physiology, pathology, and regenerative processes. Current cell-sensing modalities primarily rely on either endpoint analysis that takes static snapshots, or real-time sensing that monitors a small subset of cells."
GEMINI (Granularly Expanding Memory for Intracellular Narrative Integration) represents a novel approach to monitoring cellular dynamics in tissues. The platform employs computationally designed protein assemblies that function as intracellular memory devices, recording the history of individual cells as they respond to internal and external signals. The system grows predictably within live cells, creating tree-ring-like fluorescent patterns that enable imaging-based retrospective analysis. This technology achieves hour-level temporal accuracy in chronological information and effectively maps differential transcriptional changes, including fast dynamics occurring within 15-minute intervals. GEMINI addresses limitations of current cell-sensing methods by providing spatiotemporal precision mapping of cellular activity across tissues, advancing understanding of organ physiology, pathology, and regenerative processes.
#intracellular-recording #protein-design #cellular-memory #spatiotemporal-imaging #transcriptional-dynamics
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